Sequences Flanking Hypersensitive Sites of the β-Globin Locus Control Region Are Required for Synergistic Enhancement
نویسندگان
چکیده
منابع مشابه
Nucleosome and transcription activator antagonism at human β-globin locus control region DNase I hypersensitive sites
Locus control regions are regulatory elements that activate distant genes and typically consist of several DNase I hypersensitive sites coincident with clusters of transcription activator binding sites. To what extent nucleosomes and activators occupy these sites together or exclusively has not been extensively studied in vivo. We analyzed the chromatin structure of human beta-globin locus cont...
متن کاملReconstitution of human beta-globin locus control region hypersensitive sites in the absence of chromatin assembly.
The human beta-globin genes are regulated by the locus control region (LCR), an element composed of multiple DNase I-hypersensitive sites (HS sites) located 5' to the genes. Various functional studies indicate that the LCR confers high-level, position-independent, and copy number-dependent expression to linked globin genes in transgenic mice. However, the structural basis for LCR function is un...
متن کاملSynergism between hypersensitive sites confers long-range gene activation by the beta-globin locus control region.
The human beta-globin locus control region (LCR) consists of four erythroid-specific DNaseI hypersensitive sites (HSs) at the 5' end of the beta-globin cluster. The LCR functions over a long distance on chromosome 11 to regulate transcription and replication of the beta-globin genes. To determine whether the HSs function independently or as an integrated unit, we analyzed the requirements for l...
متن کاملSequences in the A – intergenic region are not required for stage-specific regulation of the human -globin gene locus
The human -globin locus has been extensively studied as a model of tissue and developmental stage-specific gene expression. Structural mapping of naturally occurring mutations, including transfection and transgenic studies, and the recent finding of intergenic transcripts have suggested that there are cis-acting sequence elements in the A – intergenic region involved in regulating and -globin g...
متن کاملEvidence for a locus activation region: the formation of developmentally stable hypersensitive sites in globin-expressing hybrids.
We have analyzed the chromatin structure of the human beta-globin locus in somatic cell hybrids resulting from the fusion of human non-erythroid cells and mouse erythroleukemia (MEL) cells. In these hybrids, the human adult beta-globin gene, but neither the embryonic nor fetal globin genes, is activated transcriptionally. In addition, the DNase I-resistant beta-like globin locus characteristic ...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2001
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.21.9.2969-2980.2001